PF4 Protein (AA 32-101) (Fc Tag)
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- Target See all PF4 Proteins
- PF4 (Platelet Factor 4 (PF4))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 32-101
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This PF4 protein is labelled with Fc Tag.
- Purpose
- Human CXCL4 Protein
- Sequence
- Glu32-Ser101
- Characteristics
- Recombinant Human CXCL4 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Glu32-Ser101.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CXCL4, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CXCL4 Antibody, hFc Tag with the EC50 of 6ug/ml determined by ELISA. See testing image for detail.
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Platelet Factor 4 (PF4) (AA 30-105) protein (Fc Tag)
PF4 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Platelet Factor 4 (PF4) (AA 32-101) protein (His-Avi Tag)PF4 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE
Platelet Factor 4 (PF4) (AA 32-101) protein (His-Avi Tag,Biotin)PF4 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE
Platelet Factor 4 (PF4) (AA 1-101) protein (GST tag)PF4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Platelet Factor 4 (PF4) (AA 31-101) protein (GST tag)PF4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Platelet Factor 4 (PF4) (AA 48-101) protein (His tag)Crystallography grade PF4 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Platelet Factor 4 (PF4) (AA 32-101) protein (His tag)Crystallography grade PF4 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- PF4 (Platelet Factor 4 (PF4))
- Alternative Name
- CXCL4 (PF4 Products)
- Synonyms
- CXCL4 Protein, PF-4 Protein, SCYB4 Protein, Cxcl4 Protein, Scyb4 Protein, Pf4a Protein, RATPF4A Protein, PF4A Protein, CXCL4L1 Protein, CXCL4V1 Protein, PF4-ALT Protein, SCYB4V1 Protein, PF4 Protein, platelet factor 4 Protein, platelet factor 4 variant 1 Protein, PF4 Protein, Pf4 Protein, LOC703451 Protein, PF4V1 Protein, LOC100524373 Protein, LOC102181854 Protein, LOC100630489 Protein
- Background
- Chemokines regulate leukocyte migration during physiological and pathological conditions. It is currently accepted that these chemotactic cytokines are also important in the development and progression of cancer. CXCL4 and its non-allelic variant CXCL4L1 are two platelet-associated chemokines that have been attributed anti-tumoral activity as a result of their angiostatic potential and the chemotactic activity for anti-tumoral leukocytes.
- Molecular Weight
- 34.5 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
- UniProt
- P02776
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