PF4 Protein (AA 32-101) (His-Avi Tag)

Catalog No. ABIN7274447

Recombinant PF4 protein expressed in Escherichia coli (E. coli).

Quick Overview for PF4 Protein (AA 32-101) (His-Avi Tag) (ABIN7274447)

Target: PF4 (Platelet Factor 4 (PF4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 32-101

Purification tag / Conjugate: This PF4 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human CXCL4 Protein

Sequence: Glu32-Ser101

Characteristics: Recombinant Human CXCL4 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.It contains Glu32-Ser101.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human CXCL4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in 4 mM HCL. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 4 mM HCl. Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: PF4 (Platelet Factor 4 (PF4))

Alternative Name: CXCL4

Background: Chemokines regulate leukocyte migration during physiological and pathological conditions. It is currently accepted that these chemotactic cytokines are also important in the development and progression of cancer. CXCL4 and its non-allelic variant CXCL4L1 are two platelet-associated chemokines that have been attributed anti-tumoral activity as a result of their angiostatic potential and the chemotactic activity for anti-tumoral leukocytes.

Molecular Weight: 10.68 kDa same as Bis-Tris PAGE result.

NCBI Accession: NP_002610