DKK3 Protein (AA 23-350) (His-Avi Tag)
Quick Overview for DKK3 Protein (AA 23-350) (His-Avi Tag) (ABIN7274470)
Target
Protein Type
Origin
Source
Purity
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Protein Characteristics
- AA 23-350
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Purification tag / Conjugate
- This DKK3 protein is labelled with His-Avi Tag.
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Purpose
- Human DKK3 Protein
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Sequence
- Pro23-Ile350
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Characteristics
- Recombinant Human DKK3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Pro23-Ile350.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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DKK3 (AA 23-350) protein (His-Avi Tag,Biotin)
Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
DKK3 (AA 1-350) protein (GST tag)Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
DKK3 (AA 22-350) protein (His tag)Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90% by SDS-PAGE SDS
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- DKK3
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Background
- Dkk-3, also known as REIC (Reduced Expansion in Immortalized Cells), is one of four numbered members of the Dickkopf family of Wnt antagonists.DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
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Molecular Weight
- 39.5 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
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UniProt
- Q9UBP4
Target
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