FOLR1 Protein (AA 25-233) (His-Avi Tag)

Catalog No. ABIN7274667

This Recombinant FOLR1 protein is produced in HEK-293 Cells.

Quick Overview for FOLR1 Protein (AA 25-233) (His-Avi Tag) (ABIN7274667)

Target: FOLR1 (Folate Receptor 1 (Adult) (FOLR1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE, > 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-233

Purification tag / Conjugate: This FOLR1 protein is labelled with His-Avi Tag.

Purpose: Human FOLR1 Protein

Sequence: Arg25-Met233

Characteristics: Recombinant Human FOLR1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus., It contains Arg25-Met233.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human FOLR1, His Tag at 0.5μg/ml (100μl/Well) on plate.Dose response curve for Anti-FOLR1 Antibody, hFc Tag with the EC50 of 15ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: FOLR1 (Folate Receptor 1 (Adult) (FOLR1))

Alternative Name: FOLR1

Background: Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37 - 42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown. FOLR1 binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH .

Molecular Weight: 27.5 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.

UniProt: P15328

Pathways: Dicarboxylic Acid Transport