TNFSF15 Protein (AA 91-251, Trimer) (His-DYKDDDDK Tag)

Catalog No. ABIN7274680

This Recombinant TNFSF15 protein is expressed in HEK-293 Cells.

Quick Overview for TNFSF15 Protein (AA 91-251, Trimer) (His-DYKDDDDK Tag) (ABIN7274680)

Target: TNFSF15 (Tumor Necrosis Factor (Ligand) Superfamily, Member 15 (TNFSF15))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE, > 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 91-251, Trimer

Purification tag / Conjugate: This TNFSF15 protein is labelled with His-DYKDDDDK Tag.

Purpose: Human TNFSF15 Trimer Protein

Sequence: Asp91-Leu251

Characteristics: Recombinant Human TNFSF15 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus., It contains Asp91-Leu251.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human TNFSF15 Trimer, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 18.7ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution, Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNFSF15 (Tumor Necrosis Factor (Ligand) Superfamily, Member 15 (TNFSF15))

Alternative Name: TNFSF15

Background: TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.

Molecular Weight: 58.2 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.

UniProt: O95150-1

Pathways: Positive Regulation of Endopeptidase Activity, Autophagy