TNFSF15 Protein (AA 61-252) (His-Avi Tag,Biotin)

Catalog No. ABIN7274686

This Recombinant TNFSF15 protein is expressed in HEK-293 Cells.

Quick Overview for TNFSF15 Protein (AA 61-252) (His-Avi Tag,Biotin) (ABIN7274686)

Target: TNFSF15 (Tumor Necrosis Factor (Ligand) Superfamily, Member 15 (TNFSF15))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Tris-Bis PAGE

Product Details

Protein Characteristics: AA 61-252

Purification tag / Conjugate: This TNFSF15 protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Mouse TNFSF15 Protein

Sequence: Ala61-Leu252

Characteristics: Recombinant Biotinylated Mouse TNFSF15 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ala61-Leu252.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-TNFSF15 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse TNFSF15, His Tag with the EC50 of 20.5ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNFSF15 (Tumor Necrosis Factor (Ligand) Superfamily, Member 15 (TNFSF15))

Alternative Name: TNFSF15

Background: TL1A and its functional receptor DR3 are members of the TNF/TNFR superfamilies of proteins.TL1A and DR3 are abundantly localized at inflamed intestinal areas of patients with IBD and mice with experimental ileitis or colitis and actively participate in the immunological pathways that underlie mucosal homeostasis and intestinal inflammation.Recently, an important role was demonstrated for TL1A/DR3 as potential mediators of intestinal fibrosis that is associated with the presence of gut inflammation.

Molecular Weight: 24.40 kDa. Due to glycosylation, the protein migrates to 28-38 kDa based on Tris-Bis PAGE result.

UniProt: Q5UBV8

Pathways: Positive Regulation of Endopeptidase Activity, Autophagy