Galectin 3 Protein (LGALS3) (AA 2-250) (His tag)
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- Target See all Galectin 3 (LGALS3) Proteins
- Galectin 3 (LGALS3)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-250
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Galectin 3 protein is labelled with His tag.
- Purpose
- Human Galectin 3 Protein
- Sequence
- Ala2-Ile250
- Characteristics
- Recombinant Human Galectin 3 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Ala2-Ile250.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human Galectin 3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Galectin 3 Antibody, hFc Tag with the EC50 of 17.3ng/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product LGALS3 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in 50 mM HEPES, 150 mM NaCl, 5 mM EDTA, 2 mM TCEP ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Preservative
- Other preservative
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Galectin 3 (LGALS3)
- Alternative Name
- Galectin 3 (LGALS3 Products)
- Target Type
- Chemical
- Background
- Human Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is classified as a chimeric member of the Galectin superfamily and contains one carbohydrate recognition domain (CRD) linked to a nonlectin domain.Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity).
- Molecular Weight
- 27.1 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.
- UniProt
- P17931
- Pathways
- RTK Signaling
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