CD42b Protein (AA 17-531) (His tag)
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- Target See all CD42b (GP1BA) Proteins
- CD42b (GP1BA) (Glycoprotein Ib (Platelet), alpha Polypeptide (GP1BA))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 17-531
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CD42b protein is labelled with His tag.
- Purpose
- Human CD42b/GP1BA Protein
- Sequence
- His17-Leu531
- Characteristics
- Recombinant Human CD42b/GP1BA Protein is expressed from HEK293 with His tag at the C-Terminus.It contains His17-Leu531.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CD42b, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD42b Antibody, hFc Tag with the EC50 of 8.9ng/ml determined by ELISA. See testing image for detail.
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Glycoprotein Ib (Platelet), alpha Polypeptide (GP1BA) (AA 1-626) protein (GST tag)
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Glycoprotein Ib (Platelet), alpha Polypeptide (GP1BA) (AA 17-282) protein (S tag,His tag)GP1BA Origin: Mouse Host: Escherichia coli (E. coli) Recombinant ≥ 92 % SDS, ELISA, WB, IP
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- CD42b (GP1BA) (Glycoprotein Ib (Platelet), alpha Polypeptide (GP1BA))
- Alternative Name
- CD42b (GP1BA Products)
- Background
- Absence of the CD42 complex leads to the Bernard-Soulier syndrome (BSS), which is a bleeding disorder characterized by thrombocytopenia and giant platelets. So far, mutations have been found in the genes encoding CD42a, CD42b and CD42c in patients with BSS**.
- Molecular Weight
- 57.6 kDa. Due to glycosylation, the protein migrates to 110-115 kDa based on Tris-Bis PAGE result.
- UniProt
- P07359
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