GPA33 Protein (His-Avi Tag,Biotin)
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- Target See all GPA33 Proteins
- GPA33 (Glycoprotein A33 (Transmembrane) (GPA33))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This GPA33 protein is labelled with His-Avi Tag,Biotin.
- Sequence
- Ile22-Val235
- Purity
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Anti-GPA33 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GPA33, His Tag with the EC50 of 11.0ng/ml determined by ELISA. See testing image for detail.
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- Discover our top product GPA33 Protein
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Glycoprotein A33 (Transmembrane) (GPA33) (AA 22-235) protein (His-Avi Tag)
GPA33 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Glycoprotein A33 (Transmembrane) (GPA33) (AA 22-235) protein (His tag)GPA33 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Glycoprotein A33 (Transmembrane) (GPA33) (AA 22-235) protein (Fc Tag)GPA33 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Glycoprotein A33 (Transmembrane) (GPA33) (AA 1-319) protein (GST tag)GPA33 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Glycoprotein A33 (Transmembrane) (GPA33) (AA 22-235) protein (His tag,Biotin)GPA33 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
Glycoprotein A33 (Transmembrane) (GPA33) protein (Myc-DYKDDDDK Tag)GPA33 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- GPA33 (Glycoprotein A33 (Transmembrane) (GPA33))
- Alternative Name
- GPA33 (GPA33 Products)
- Background
- Glycoprotein A33, GPA33, A33, MGC129986, MGC129987,Glycoprotein A33 (GPA33) is also known as Cell surface A33 antigen, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95 % of colon cancers. GPA33 The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily, which contains. GPA33 may play a role in cell-cell recognition and signaling.
- Molecular Weight
- 26.5 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Tris-Bis PAGE result.
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