GPA33 Protein (AA 22-235) (His-Avi Tag)
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- Target See all GPA33 Proteins
- GPA33 (Glycoprotein A33 (Transmembrane) (GPA33))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 22-235
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This GPA33 protein is labelled with His-Avi Tag.
- Purpose
- Human GPA33/A33 Protein
- Sequence
- Ile22-Val235
- Characteristics
- Recombinant Human GPA33/A33 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile22-Val235.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human GPA33, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-GPA33 Antibody, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- GPA33 (Glycoprotein A33 (Transmembrane) (GPA33))
- Alternative Name
- GPA33 (GPA33 Products)
- Synonyms
- A33 Protein, 2010310L10Rik Protein, 2210401D16Rik Protein, BB116197 Protein, glycoprotein A33 Protein, glycoprotein A33 (transmembrane) Protein, GPA33 Protein, Gpa33 Protein
- Background
- Glycoprotein A33 (GPA33) is also known as Cell surface A33 antigen, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95 % of colon cancers. GPA33 The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily, which contains. GPA33 may play a role in cell-cell recognition and signaling.
- Molecular Weight
- 26.5 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Tris-Bis PAGE result.
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