GPA33 Protein (AA 22-235) (His tag,Biotin)
Quick Overview for GPA33 Protein (AA 22-235) (His tag,Biotin) (ABIN2444135)
Target
See all GPA33 ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 22-235
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Purification tag / Conjugate
- This GPA33 protein is labelled with His tag,Biotin.
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Brand
- MABSol®,UltraLys
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Sequence
- AA 22-235
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Specificity
- The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
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Characteristics
- This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 24.5 kDa. The protein migrates as 35-40 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
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Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
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Glycoprotein A33 (Transmembrane) (GPA33) (AA 22-235) (Active) protein (His-Avi Tag)
GPA33 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Glycoprotein A33 (Transmembrane) (GPA33) (AA 22-235) (Active) protein (Biotin,His-Avi Tag)GPA33 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Glycoprotein A33 (Transmembrane) (GPA33) (AA 22-235) (Active) protein (His tag)GPA33 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Glycoprotein A33 (Transmembrane) (GPA33) (AA 1-319) protein (GST tag)GPA33 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Glycoprotein A33 (Transmembrane) (GPA33) (AA 22-235) protein (Fc Tag)GPA33 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
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Comment
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A chemically labeled biotinylated protein with ultra sensitivity.
The product is produced using a chemical labeling approach. The primary amines in the side chains of lysine residues and the N-terminus of protein are conjugated with biotins.
Chemical labeling usually results in multiple biotin attachment on a single protein molecule, which could potentially lead to higher detection sensitivity. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- PBS, pH 7.4
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Handling Advice
- Please avoid repeated freeze-thaw cycles.
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Storage
- -20 °C
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Storage Comment
- Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.
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- GPA33 (Glycoprotein A33 (Transmembrane) (GPA33))
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Alternative Name
- GPA33
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Background
- Glycoprotein A33 (GPA33) is also known as Cell surface A33 antigen, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95 % of colon cancers. GPA33 The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily, which contains. GPA33 may play a role in cell-cell recognition and signaling.
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Molecular Weight
- 24.4 kDa
Target
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