GPC1 Protein (AA 24-529) (His tag)

Catalog No. ABIN7274776

Product Details

Target: GPC1 (Glypican 1 (GPC1))

Protein Type: Recombinant

Protein Characteristics: AA 24-529

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This GPC1 protein is labelled with His tag.

Purpose: Mouse Glypican 1/GPC1 Protein

Sequence: Asp24-Ser529

Characteristics: Recombinant Mouse Glypican 1/GPC1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp24-Ser529.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in 50 mM Tris,150 mM NaCl ( pH 7.5). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: GPC1 (Glypican 1 (GPC1))

Alternative Name: Glypican 1 (GPC1 Products)

Synonyms: gpc1 Protein, zgc:63947 Protein, GPC1 Protein, Glypican-1 Protein, glypican Protein, AI462976 Protein, HSPG M12 Protein, HSPGM12 Protein, glypican 1b Protein, glypican 1 Protein, gpc1b Protein, GPC1 Protein, gpc1 Protein, Gpc1 Protein

Background: CAR-T cells targeting glypican-1 (GPC1)-specific human and murine CAR-T cells generated from our original anti-human/mouse GPC1 antibody showed strong antitumor effects in xenogeneic and syngeneic mouse models, respectively. Importantly, the murine CAR-T cells enhanced endogenous T cell responses against a non-GPC1 tumor antigen through the mechanism of antigen-spreading and showed synergistic antitumor effects with anti-PD-1 antibody without any adverse effects in syngeneic models.

Molecular Weight: 56.8 kDa. Due to glycosylation, the protein migrates to 65-68 kDa based on Tris-Bis PAGE result.

UniProt: Q9QZF2

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation