GPC1 Protein (AA 24-530) (His tag)
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- Target See all GPC1 Proteins
- GPC1 (Glypican 1 (GPC1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 24-530
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This GPC1 protein is labelled with His tag.
- Purpose
- Human Glypican 1/GPC1 Protein
- Sequence
- Asp24-Ser530
- Characteristics
- Recombinant Human Glypican 1/GPC1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp24-Ser530.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human FGF basic at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 21.9ng/ml determined by ELISA. See testing image for detail.
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Glypican 1 (GPC1) (AA 24-529) protein (His tag)
GPC1 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Glypican 1 (GPC1) (Active) protein (His tag)GPC1 Origin: Mouse Host: Human Cells Recombinant > 90 % as determined by SDS-PAGE Active
Glypican 1 (GPC1) (AA 24-131) protein (GST tag)GPC1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Glypican 1 (GPC1) (AA 71-165) protein (GST tag)GPC1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Glypican 1 (GPC1) (AA 24-530) protein (His tag)Crystallography grade GPC1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Glypican 1 (GPC1) (AA 24-529) protein (His tag)Crystallography grade GPC1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Glypican 1 (GPC1) (AA 24-529) (Active) protein (His tag)GPC1 Origin: Mouse Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Glypican 1 (GPC1) (AA 24-530) (Active) protein (His tag)GPC1 Origin: Human Host: HEK-293 Cells Recombinant >85 % as determined by SDS-PAGE. Active
Glypican 1 (GPC1) (AA 24-530) (Active) protein (Fc Tag)GPC1 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- GPC1 (Glypican 1 (GPC1))
- Alternative Name
- Glypican 1 (GPC1 Products)
- Synonyms
- gpc1 Protein, zgc:63947 Protein, GPC1 Protein, Glypican-1 Protein, glypican Protein, AI462976 Protein, HSPG M12 Protein, HSPGM12 Protein, glypican 1b Protein, glypican 1 Protein, gpc1b Protein, GPC1 Protein, gpc1 Protein, Gpc1 Protein
- Background
- CAR-T cells targeting glypican-1 (GPC1)-specific human and murine CAR-T cells generated from our original anti-human/mouse GPC1 antibody showed strong antitumor effects in xenogeneic and syngeneic mouse models, respectively. Importantly, the murine CAR-T cells enhanced endogenous T cell responses against a non-GPC1 tumor antigen through the mechanism of antigen-spreading and showed synergistic antitumor effects with anti-PD-1 antibody without any adverse effects in syngeneic models.
- Molecular Weight
- 57.1 kDa. Due to glycosylation, the protein migrates to 70-135 kDa based on Tris-Bis PAGE result.
- Pathways
- Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation
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