ERBB3 Protein (AA 20-641) (His tag)

Catalog No. ABIN7274795

Product Details

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Protein Type: Recombinant

Protein Characteristics: AA 20-641

Origin: Cynomolgus, Rhesus Monkey

Source: HEK-293 Cells

Purification tag / Conjugate: This ERBB3 protein is labelled with His tag.

Purpose: Cynomolgus/Rhesus macaque Her3 Protein

Sequence: Ser20-His641

Characteristics: Recombinant Cynomolgus/Rhesus macaque Her3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser20-His641.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus/Rhesus macaque Her3, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-Her3 Antibody, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Alternative Name: Her3 (ERBB3 Products)

Synonyms: ErbB-3 Protein, HER3 Protein, LCCS2 Protein, MDA-BF-1 Protein, c-erbB-3 Protein, c-erbB3 Protein, erbB3-S Protein, p180-ErbB3 Protein, p45-sErbB3 Protein, p85-sErbB3 Protein, ERBB3 Protein, C76256 Protein, Erbb-3 Protein, Erbb3r Protein, Her3 Protein, nuc-ErbB3 Protein, erb-b2 receptor tyrosine kinase 3 Protein, v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) Protein, ERBB3 Protein, Erbb3 Protein

Background: Her3, also called ErbB3, is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors.Her3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric Her3 serves as a low affinity receptor for the heregulins (HRG).

Molecular Weight: 69.54 kDa. Due to glycosylation, the protein migrates to 85-105 kDa based on Tris-Bis PAGE result.

NCBI Accession: XP_005571238

UniProt: G7N7B1

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway