HLAG Protein (Tetramer) (HLA-G)
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- Target See all HLAG Proteins
- HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))
- Protein Type
- Recombinant
- Protein Characteristics
- Tetramer
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Origin
- Rhesus Monkey
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This HLAG protein is labelled with HLA-G.
- Purpose
- Rhesus macaque HLA-G & B2M & Peptide (RIIPRHLQL) Tetramer Protein
- Sequence
- Gly25-Thr305 (HLA-G), Ile21-Met119 (B2M) and RIIPRHLQL peptide
- Specificity
- Uni-Prot: O02948 (HLA-G), Q6V7J5 (B2M), RIIPRHLQL
- Characteristics
- Recombinant Rhesus macaque HLA-G & B2M & Peptide (RIIPRHLQL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-G),Ile21-Met119(B2M) and RIIPRHLQL peptide.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- The affinity constant of 1.74 nM as determined in SPR assay (Biacore T200). See testing image for detail.
- Top Product
- Discover our top product HLAG Protein
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HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (Tetramer) protein (HLA-G)
HLAG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (Tetramer) protein (HLA-G)HLAG Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (Tetramer) protein (Biotin,HLA-G)HLAG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (Monomer) protein (Biotin,HLA-G)HLAG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (Monomer) protein (HLA-G)HLAG Origin: Rhesus Monkey Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (Monomer) protein (HLA-G)HLAG Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (Monomer) protein (HLA-G)HLAG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (Tetramer) protein (PE,HLA-G)HLAG Origin: Human Host: HEK-293 Cells Recombinant
HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (AA 1-338) protein (GST tag)HLAG Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (AA 25-337), (partial) protein (GST tag)HLAG Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))
- Alternative Name
- HLA-G (HLAG Products)
- Background
- HLA-G is a molecule that was first known to confer protection to the fetus from destruction by the immune system of its mother, thus critically contributing to fetal-maternal tolerance. The first functional finding constituted the basis for HLA-G research and can be summarized as such: HLA-G, membrane-bound or soluble, strongly binds its inhibitory receptors on immune cells (NK, T, B, monocytes/dendritic cells), inhibits the functions of these effectors, and so induces immune inhibition.
- Molecular Weight
- 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
- UniProt
- O02948
- Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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