HMGB1 Protein (AA 1-215) (His tag,Biotin)

Catalog No. ABIN7274823

Product Details

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Protein Type: Recombinant

Protein Characteristics: AA 1-215

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This HMGB1 protein is labelled with His tag,Biotin.

Purpose: Biotinylated Human HMGB1 Protein (Primary Amine Labeling)

Sequence: Met1-Glu215

Characteristics: Recombinant Biotinylated Human HMGB1 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Met1-Glu215.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: HMGB1 (HMGB1 Products)

Background: High-mobility group box 1 (HMGB1) is a ubiquitous nuclear protein that promotes inflammation when released extracellularly after cellular activation, stress, damage or death. HMGB1 operates as one of the most intriguing molecules in inflammatory disorders via recently elucidated signal and molecular transport mechanisms. Treatments based on antagonists specifically targeting extracellular HMGB1 have generated encouraging results in a wide number of experimental models of infectious and sterile inflammation.

Molecular Weight: 26 kDa. Due to glycosylation, the protein migrates to 32-36 kDa based on Tris-Bis PAGE result.

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome