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IgE Protein (AA 91-421) (His tag)

This Recombinant IgE protein is produced in HEK-293 Cells.
Catalog No. ABIN7274856

Quick Overview for IgE Protein (AA 91-421) (His tag) (ABIN7274856)

Target

See all IgE Proteins
IgE

Protein Type

Recombinant

Origin

  • 9
  • 7
  • 5
  • 4
  • 2
  • 1
Mouse

Source

  • 10
  • 6
  • 4
  • 3
  • 2
  • 1
  • 1
  • 1
HEK-293 Cells

Purity

> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
  • Protein Characteristics

    AA 91-421

    Purification tag / Conjugate

    This IgE protein is labelled with His tag.

    Purpose

    Mouse IgE Protein

    Sequence

    Val91-Ser421

    Characteristics

    Recombinant Mouse IgE Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val91-Ser421.

    Sterility

    0.22 μm filtered

    Endotoxin Level

    Less than 1EU per ug by the LAL method.
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  • Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

    Buffer

    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

    Storage

    -20 °C,-80 °C

    Storage Comment

    -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

    Expiry Date

    12 months
  • Target

    IgE

    Background

    Immunoglobulin E (IgE) is well known for its role in allergic disease, the manifestations of which are mediated through its two Fc receptors, FcεRI and CD23 (FcεRII). IgE and its interactions with these receptors are therefore potential targets for therapeutic intervention, and exciting progress has been made in this direction. Furthermore, recent structural studies of IgE-Fc, the two receptors, and of their complexes, have revealed a remarkable degree of plasticity at the IgE-CD23 interface and an even more remarkable degree of dynamic flexibility within the IgE molecule.

    Molecular Weight

    38.5 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

    UniProt

    P06336
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