IgG1 Fc Protein
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- Target
- IgG1 Fc
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Sequence
- Asp103-Lys330
- Purity
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- The affinity constant of 0.283 μM as determined in SPR assay (Biacore T200). See testing image for detail.
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IgG1 Fc protein
Origin: Mouse Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
IgG1 Fc protein (Fc Tag)Origin: Mouse Host: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE
IgG1 Fc (AA 100-330) protein (DYKDDDDK Tag)Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
IgG1 Fc (AA 100-330) protein (gD Tag)Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
IgG1 Fc (AA 98-324) protein (AVI tag,Biotin)Origin: Mouse Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- IgG1 Fc
- Background
- IgG1, Igh-4, RGD1359539, VH7183,IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues.IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.
- Molecular Weight
- 25.5 kDa. Due to glycosylation, the protein migrates to 31-33 kDa based on Tris-Bis PAGE result.
- UniProt
- P01857
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