IL1RAP Protein (AA 21-359) (His-Avi Tag,Biotin)

Catalog No. ABIN7274905

Product Details

Target: IL1RAP (Interleukin 1 Receptor Accessory Protein (IL1RAP))

Protein Type: Recombinant

Protein Characteristics: AA 21-359

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This IL1RAP protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Human IL-1R3/IL-1 RAcP Protein

Sequence: Ser21-Glu359

Characteristics: Recombinant Biotinylated Human IL-1R3/IL-1 RAcP Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser21-Glu359.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human IL-1R3 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-IL-1R3 Antibody, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL1RAP (Interleukin 1 Receptor Accessory Protein (IL1RAP))

Alternative Name: IL-1R3 (IL1RAP Products)

Background: IL-1 Receptor Accessory Protein (IL-1 RAcP), also known as IL-1 R3, is a ubiquitously expressed 70-90 kDa member of the Interleukin-1 receptor family of proteins. It serves as a non-ligand-binding component of the receptors for IL-1 alpha, IL-1 beta, IL-33, and IL-36. IL-1 R3 is a coreceptor for IL1RL2 in the IL-36 signaling system. IL-1 R3 is a coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways.

Molecular Weight: 42 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Tris-Bis PAGE result.

UniProt: Q9NPH3-1

Pathways: NF-kappaB Signaling, Growth Factor Binding