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IL-21 Protein (AA 32-162)

IL21 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE
Catalog No. ABIN7274916
  • Target See all IL-21 (IL21) Proteins
    IL-21 (IL21) (Interleukin 21 (IL21))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 32-162
    Origin
    • 37
    • 16
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 33
    • 16
    • 6
    • 4
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Purpose
    Human IL-21 Protein
    Sequence
    Gln32-Ser162
    Characteristics
    Recombinant Human IL-21 Protein is expressed from E.coli without tag.It contains Gln32-Ser162.
    Purity
    > 95 % as determined by Tris-Bis PAGE
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Human IL-21, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human IL-21R, hFc Tag with the EC50 of 63.6ng/ml determined by ELISA. See testing image for detail.
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 20 mM NaAc, 150 mM NaCl ( pH 5.5).
    Buffer
    Lyophilized from 0.22μm filtered solution in 20 mM NaAc, 150 mM NaCl ( pH 5.5). Normally 8 % trehalose is added as protectant before lyophilization.
    Storage
    -20 °C,-80 °C
    Storage Comment
    -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    IL-21 (IL21) (Interleukin 21 (IL21))
    Alternative Name
    IL-21 (IL21 Products)
    Background
    Interleukin-21 (IL-21), produced predominantly by CD4 T cells and natural killer T (NKT) cells, is a newly discovered member of the common γ-chain family of cytokines. It has been implicated in many immunological processes and has been linked to autoimmune diseases, allergies and other inflammatory diseases.
    Molecular Weight
    15.4 kDa same as Tris-Bis PAGE result.
    UniProt
    Q9HBE4-1
    Pathways
    JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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