IL-21 Protein (AA 32-162) (His-Avi Tag,Biotin)
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- Target See all IL-21 (IL21) Proteins
- IL-21 (IL21) (Interleukin 21 (IL21))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 32-162
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This IL-21 protein is labelled with His-Avi Tag,Biotin.
- Purpose
- Biotinylated Human IL-21 Protein
- Sequence
- Gln32-Ser162
- Characteristics
- Recombinant Biotinylated Human IL-21 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.It contains Gln32-Ser162.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-21R, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human IL-21, His Tag with the EC50 of 3.5ng/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product IL21 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 20 mM NaAc, 500 mM NaCl ( pH 5.5).
- Buffer
- Lyophilized from 0.22 μm filtered solution in 20 mM NaAc, 500 mM NaCl ( pH 5.5). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- IL-21 (IL21) (Interleukin 21 (IL21))
- Alternative Name
- IL-21 (IL21 Products)
- Background
- Interleukin-21 (IL-21), produced predominantly by CD4 T cells and natural killer T (NKT) cells, is a newly discovered member of the common γ-chain family of cytokines. It has been implicated in many immunological processes and has been linked to autoimmune diseases, allergies and other inflammatory diseases.
- Molecular Weight
- 18.30 kDa same as Tris-Bis PAGE result.
- Pathways
- JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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