IL18BP Protein (AA 31-194) (Fc Tag)
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- Target See all IL18BP Proteins
- IL18BP (Interleukin 18 Binding Protein (IL18BP))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 31-194
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This IL18BP protein is labelled with Fc Tag.
- Purpose
- Human IL-18BP Protein
- Sequence
- Thr31-Gly194
- Characteristics
- Recombinant Human IL-18BP Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Thr31-Gly194.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-18 at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 18.2ng/ml determined by ELISA. The affinity constant of 0.04nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Interleukin 18 Binding Protein (IL18BP) (AA 31-194) protein (His tag)
IL18BP Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 18 Binding Protein (IL18BP) (AA 31-194) protein (His-Avi Tag,Biotin)IL18BP Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 18 Binding Protein (IL18BP) (AA 29-193) protein (His tag)IL18BP Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 18 Binding Protein (IL18BP) (AA 29-193) protein (Fc Tag)IL18BP Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 18 Binding Protein (IL18BP) (AA 31-194) protein (hIgG-His-tag)IL18BP Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90% by SDS-PAGE SDS
Interleukin 18 Binding Protein (IL18BP) (AA 31-194) protein (GST tag)IL18BP Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Interleukin 18 Binding Protein (IL18BP) (AA 31-192) protein (Fc Tag)IL18BP Origin: Dog Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 18 Binding Protein (IL18BP) (AA 28-207) protein (Fc Tag)IL18BP Origin: Cynomolgus Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 18 Binding Protein (IL18BP) proteinIL18BP Origin: Human Host: Escherichia coli (E. coli) Recombinant SDS
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- IL18BP (Interleukin 18 Binding Protein (IL18BP))
- Alternative Name
- IL-18BP (IL18BP Products)
- Background
- Cytokines were the first modern immunotherapies to produce durable responses in patients with advanced cancer,components of the interleukin-18 (IL-18) pathway are upregulated on tumour-infiltrating lymphocytes, suggesting that IL-18 therapy could enhance anti-tumour immunity. IL-18BP, a high-affinity IL-18 decoy receptor, is frequently upregulated in diverse human and mouse tumours and limits the anti-tumour activity of IL-18 in mice.
- Molecular Weight
- 44.4 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Tris-Bis PAGE result.
- UniProt
- O95998-2
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