IL21 Receptor Protein (AA 20-232) (His tag)

Catalog No. ABIN7275014

Product Details

Target: IL21 Receptor (IL21R) (Interleukin 21 Receptor (IL21R))

Protein Type: Recombinant

Protein Characteristics: AA 20-232

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This IL21 Receptor protein is labelled with His tag.

Purpose: Human IL-21R Protein

Sequence: Cys20-Glu232

Characteristics: Recombinant Human IL-21R Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys20-Glu232.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-21 at 1μg/ml (100μl/well) on the plate. Dose response curve for Human IL-21R, His Tag with the EC50 of 24.3ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL21 Receptor (IL21R) (Interleukin 21 Receptor (IL21R))

Alternative Name: IL-21R (IL21R Products)

Background: IL-21 and IL-21R were highly expressed in the lesional skin and peripheral blood of psoriasis patients. IL-21 promoted CD4 T cells proliferation and Th17 cells differentiation and inhibiting Treg cells differentiation by upregulating RORγt expression and downregulating Foxp3 expression, with increased expression and secretion of IL-17A and IL-22.Microbial translocation and the associated immune activation during HIV-1 infection may lead to high expression levels of the IL-21R activation marker in RM B cells, a feature associated with increased apoptosis and a reduced number of these cells in the circulation.

Molecular Weight: 25.65 kDa. Due to glycosylation, the protein migrates to 43-50 kDa based on Tris-Bis PAGE result.

UniProt: Q9HBE5

Pathways: JAK-STAT Signaling