IL13 Receptor alpha 1 Protein (AA 27-343) (Fc Tag)

Catalog No. ABIN7275027

Product Details

Target: IL13 Receptor alpha 1 (IL13RA1) (Interleukin 13 Receptor, alpha 1 (IL13RA1))

Protein Type: Recombinant

Protein Characteristics: AA 27-343

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This IL13 Receptor alpha 1 protein is labelled with Fc Tag.

Purpose: Human IL-13Ra1 Protein

Sequence: Ala27-Thr343

Characteristics: Recombinant Human IL-13Ra1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ala27-Thr343.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-13 at 1μg/ml (100μl/Well). Dose response curve for Human IL-13Ra1, hFc Tag with the EC50 of 2.7μg/ml determined by ELISA. The affinity constant of 53.1nM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL13 Receptor alpha 1 (IL13RA1) (Interleukin 13 Receptor, alpha 1 (IL13RA1))

Alternative Name: IL-13Ra1 (IL13RA1 Products)

Background: Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.

Molecular Weight: 63.4 kDa. Due to glycosylation, the protein migrates to 78-90 kDa based on Tris-Bis PAGE result.

UniProt: P78552

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response