LRRC15 Protein (AA 22-536) (His tag)

Catalog No. ABIN7275217

Product Details

Target: LRRC15 (Leucine Rich Repeat Containing 15 (LRRC15))

Protein Type: Recombinant

Protein Characteristics: AA 22-536

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This LRRC15 protein is labelled with His tag.

Purpose: Mouse LRRC15/LIB Protein

Sequence: Tyr22-Gly536

Characteristics: Recombinant Mouse LRRC15/LIB Protein is expressed from HEK293 with His tag at the C-Terminus., It contains Tyr22-Gly536.

Purity: > 95 % as determined by Tris-Bis PAGE, > 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3 months after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: LRRC15 (Leucine Rich Repeat Containing 15 (LRRC15))

Alternative Name: LRRC15 (LRRC15 Products)

Background: The chimeric protein binds in vitro and in vivo to a specific element upstream of LRRC15, leading to dramatic transcriptional activation.  LRRC15 encodes a leucine-rich transmembrane protein, present at the leading edge of migrating cells, the expression of which in normal tissues is restricted to the invasive cytotrophoblast layer of the placenta, small interfering (siRNA)-mediated suppression of LRRC15 expression in breast cancer cells leads to abrogation of invasiveness in vitro.

Molecular Weight: 58.56 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

UniProt: Q80X72