CCL2 Protein (AA 24-99) (His-Avi Tag)

Catalog No. ABIN7275240

This Recombinant CCL2 protein is produced in HEK-293 Cells.

Quick Overview for CCL2 Protein (AA 24-99) (His-Avi Tag) (ABIN7275240)

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 24-99

Purification tag / Conjugate: This CCL2 protein is labelled with His-Avi Tag.

Purpose: Human MCP-1/CCL2 Protein

Sequence: Gln24-Thr99

Characteristics: Recombinant Human MCP-1/CCL2 is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln24-Thr99.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Alternative Name: MCP-1

Background: Monocyte chemotactic protein-1 (MCP-1) (also referred to as chemokine (C-C motif) ligand 2 (CCL2) is expressed by mainly inflammatory cells and endothelial cells. MCP-1 has been reported to play an important role in the pathogenesis of atherosclerosis and considerable evidence supports that the monocyte containing MCPs and macrophage influences the growth of other cell types within the atherosclerotic lesion. This review will focus on the general structure features of MCP-1 and its role in atherosclerosis.

Molecular Weight: 11.5 kDa. Due to glycosylation, the protein migrates to 15-20 kDa based on Tris-Bis PAGE result.

UniProt: P13500

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection