PVRL1 Protein (AA 31-334) (His-Avi Tag)
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- Target See all PVRL1 Proteins
- PVRL1 (Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 31-334
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This PVRL1 protein is labelled with His-Avi Tag.
- Purpose
- Human Nectin-1/PVRL1/CD111 Protein
- Sequence
- Gln31-Thr334
- Characteristics
- Recombinant Human Nectin-1/PVRL1/CD111 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln31-Thr334.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- The affinity constant of 0.17μM as determined in SPR assay (Biacore T200). See testing image for detail.
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Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1) (AA 31-334) protein (His-Avi Tag,Biotin)
PVRL1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1) (AA 31-334) (Active) protein (Fc Tag)PVRL1 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by reduced SDS-PAGE. Active
Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1) (AA 31-355) protein (His tag)PVRL1 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 95% by SDS-PAGE SDS
Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1) (AA 1-352) protein (GST tag)PVRL1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1) protein (His tag)PVRL1 Origin: Rat Host: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE
Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1) protein (Fc Tag)PVRL1 Origin: Rat Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- PVRL1 (Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1))
- Alternative Name
- Nectin-1 (PVRL1 Products)
- Background
- Nectins are a small family of Ca -independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that organize intercellular junctions. They are highly homologous to the human receptor for poliovirus, and as such have been alternately named poliovirus receptor-related proteins.Nectin-1 promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between Nectin-1 and Nectin-3 and between Nectin-1 and Nectin-4. Has some neurite outgrowth-promoting activity.
- Molecular Weight
- 36.9 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Tris-Bis PAGE result.
- UniProt
- Q15223-1
- Pathways
- Cell-Cell Junction Organization
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