Notch1 Protein (AA 19-526) (His-Avi Tag)
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- Target See all Notch1 (NOTCH1) Proteins
- Notch1 (NOTCH1) (Notch 1 (NOTCH1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 19-526
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Notch1 protein is labelled with His-Avi Tag.
- Purpose
- Human Notch 1 Protein
- Sequence
- Ala19-Gln526
- Characteristics
- Recombinant Human Notch 1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala19-Gln526.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- The affinity constant of 0.48 μM as determined in SPR assay (Biacore T200). See testing image for detail.
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Notch 1 (NOTCH1) protein (His-Avi Tag,Biotin)
NOTCH1 Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Notch 1 (NOTCH1) protein (His tag)NOTCH1 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Notch 1 (NOTCH1) (AA 2309-2555) protein (His tag)NOTCH1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Notch 1 (NOTCH1) (AA 23-132) protein (GST tag)NOTCH1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Notch 1 (NOTCH1) (Active) protein (His tag)NOTCH1 Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 82 % as determined by SDS-PAGE Active
Notch 1 (NOTCH1) (AA 19-526) (Active) protein (Fc Tag)NOTCH1 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Notch 1 (NOTCH1) (AA 19-526) (Active) protein (His tag)NOTCH1 Origin: Mouse Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Notch1 (NOTCH1) (Notch 1 (NOTCH1))
- Alternative Name
- Notch 1 (NOTCH1 Products)
- Background
- Human Notch-1 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes.Notch-1 functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
- Molecular Weight
- 56.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
- UniProt
- P46531
- Pathways
- Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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