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PD-L1 Protein (AA 19-238) (His tag)

PD-L1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Catalog No. ABIN7275429
  • Target See all PD-L1 Proteins
    PD-L1 (CD274 (PD-L1))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 19-238
    Origin
    • 37
    • 15
    • 6
    • 5
    • 4
    • 3
    • 1
    Human
    Source
    • 41
    • 9
    • 7
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This PD-L1 protein is labelled with His tag.
    Purpose
    Human PD-L1/B7-H1 Protein
    Sequence
    Phe19-Arg238
    Characteristics
    Recombinant Human PD-L1/B7-H1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe19-Arg238.
    Purity
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Human PD-L1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA. See testing image for detail.
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    Discover our top product PD-L1 Protein
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    Buffer
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    Storage
    -20 °C,-80 °C
    Storage Comment
    -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    PD-L1 (CD274 (PD-L1))
    Alternative Name
    PD-L1 (PD-L1 Products)
    Synonyms
    B7-H Protein, B7H1 Protein, PD-L1 Protein, PDCD1L1 Protein, PDCD1LG1 Protein, PDL1 Protein, A530045L16Rik Protein, B7h1 Protein, Pdcd1l1 Protein, Pdcd1lg1 Protein, Pdl1 Protein, RGD1566211 Protein, CD274 molecule Protein, CD274 antigen Protein, programmed cell death 1 ligand 2 Protein, CD274 Protein, Cd274 Protein, PDCD1LG2 Protein
    Background
    B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.
    Molecular Weight
    26 kDa. Due to glycosylation, the protein migrates to 35-42 kDa based on Tris-Bis PAGE result.
    Pathways
    Cancer Immune Checkpoints
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