PD-L1 Protein (AA 19-238) (Biotin,His-Avi Tag)

Catalog No. ABIN7275434

Recombinant PD-L1 protein expressed in HEK-293 Cells.

Quick Overview for PD-L1 Protein (AA 19-238) (Biotin,His-Avi Tag) (ABIN7275434)

Target: PD-L1 (CD274 (PD-L1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 19-238

Purification tag / Conjugate: This PD-L1 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human PD-L1/B7-H1 Protein

Sequence: Phe19-Arg238

Characteristics: Recombinant Biotinylated Human PD-L1/B7-H1 is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe19-Arg238.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human PD-L1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human PD-1, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA (QC Test).,Immobilized Anti-PD-L1 Antibody, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human PD-L1, His Tag with the EC50 of 9.4ng/ml determined by ELISA.,Biotinylated Human PD-L1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Serial dilutions of Anti-PD-1 Antibody were added into Human PD-1, hFc Tag: Biotinylated Human PD-L1, His Avi Tag binding reactions. The half maximal inhibitiory concentration (IC50) is 0.22ug/ml.,Loaded Biotinylated Human PD-L1, His Tag on Streptavidin-Biosensor can bind Human PD-1, His Tag with an affinity constant of 0.94 μM as determined in BLI assay (Gator® Prime).,The purity of Biotinylated Human PD-L1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: PD-L1

Background: B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.

Molecular Weight: 28.1 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints