PDGFRB Protein (AA 33-530) (His-Avi Tag)

Catalog No. ABIN7275445

This Recombinant PDGFRB protein is expressed in HEK-293 Cells.

Quick Overview for PDGFRB Protein (AA 33-530) (His-Avi Tag) (ABIN7275445)

Target: PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 33-530

Purification tag / Conjugate: This PDGFRB protein is labelled with His-Avi Tag.

Purpose: Human PDGF R beta/CD140b Protein

Sequence: Leu33-Phe530

Characteristics: Recombinant Human PDGF R beta/CD140b Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu33-Phe530.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

Alternative Name: PDGF R beta

Background: PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA,PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. PDGF R plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites.

Molecular Weight: 59 kDa. Due to glycosylation, the protein migrates to 78-115 kDa based on Tris-Bis PAGE result.

UniProt: P09619

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling