RNF34 Protein (AA 24-197) (Fc Tag)
Quick Overview for RNF34 Protein (AA 24-197) (Fc Tag) (ABIN7275519)
Target
See all RNF34 ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 24-197
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Purification tag / Conjugate
- This RNF34 protein is labelled with Fc Tag.
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Purpose
- Human RNF43 Protein
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Sequence
- Gly24-Tyr197
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Characteristics
- Recombinant Human RNF43 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly24-Tyr197.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human R spondin 1, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human RNF34, hFc Tag with the EC50 of 0.4μg/ml determined by ELISA. See testing image for detail.
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Ring Finger Protein 34 (RNF34) (AA 24-197) protein (Fc Tag)
RNF34 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Ring Finger Protein 34 (RNF34) (AA 1-372) protein (His tag)custom-made RNF34 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ring Finger Protein 34 (RNF34) (AA 1-376) protein (His tag)custom-made RNF34 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ring Finger Protein 34 (RNF34) (AA 1-373) protein (His tag)RNF34 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Ring Finger Protein 34 (RNF34) (AA 1-372) protein (GST tag)RNF34 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Ring Finger Protein 34 (RNF34) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)RNF34 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- RNF34 (Ring Finger Protein 34 (RNF34))
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Alternative Name
- RNF34
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Background
- RNF43 (E3 ubiquitin-protein ligase RNF43 or RING-type E3 ubiquitin transferase RNF43) functions as a tumor suppressor, by exerting a predominant negative feedback mechanism in the Wnt/β-catenin signaling pathway. RNF43 inhibits Wnt/beta-catenin signaling by ubiquitinating Frizzled receptor and targeting it to the lysosomal pathway for degradation.
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Molecular Weight
- 45.8 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.
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UniProt
- Q68DV7-1
Target
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