RNF34 Protein (AA 24-197) (Fc Tag)
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- Target See all RNF34 Proteins
- RNF34 (Ring Finger Protein 34 (RNF34))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 24-197
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This RNF34 protein is labelled with Fc Tag.
- Purpose
- Mouse RNF43 Protein
- Sequence
- Gly24-Tyr197
- Characteristics
- Recombinant Mouse RNF43 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly24-Tyr197.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Mouse R spondin 1, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.21μg/ml determined by ELISA. See testing image for detail.
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Ring Finger Protein 34 (RNF34) (AA 24-197) protein (Fc Tag)
RNF34 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Ring Finger Protein 34 (RNF34) (AA 1-372) protein (His tag)custom-made RNF34 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ring Finger Protein 34 (RNF34) (AA 1-376) protein (His tag)custom-made RNF34 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ring Finger Protein 34 (RNF34) (AA 1-373) protein (His tag)RNF34 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Ring Finger Protein 34 (RNF34) (AA 1-372) protein (GST tag)RNF34 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Ring Finger Protein 34 (RNF34) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)RNF34 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- RNF34 (Ring Finger Protein 34 (RNF34))
- Alternative Name
- RNF34 (RNF34 Products)
- Background
- RNF43 (E3 ubiquitin-protein ligase RNF43 or RING-type E3 ubiquitin transferase RNF43) functions as a tumor suppressor, by exerting a predominant negative feedback mechanism in the Wnt/β-catenin signaling pathway. RNF43 inhibits Wnt/beta-catenin signaling by ubiquitinating Frizzled receptor and targeting it to the lysosomal pathway for degradation.
- Molecular Weight
- 45.6 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result.
- UniProt
- Q5NCP0
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