Sclerostin Protein (SOST) (AA 24-213) (His-Avi Tag,Biotin)

Catalog No. ABIN7275637

This Recombinant Sclerostin protein is expressed in HEK-293 Cells.

Quick Overview for Sclerostin Protein (SOST) (AA 24-213) (His-Avi Tag,Biotin) (ABIN7275637)

Target: Sclerostin (SOST)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE

Product Details

Protein Characteristics: AA 24-213

Purification tag / Conjugate: This Sclerostin protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Human SOST/Sclerostin Protein

Sequence: Gln24-Tyr213

Characteristics: Recombinant Biotinylated Human SOST/Sclerostin Protein is expressed from HEK293 with His and Avi tag at the N-Terminus.It contains Gln24-Tyr213.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human SOST, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-SOST Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Sclerostin (SOST)

Alternative Name: SOST

Background: SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. SOST is negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.

Molecular Weight: 24.2 kDa. Due to glycosylation,the protein migrates to 28-40 kDa based on Tris-Bis PAGE result.

UniProt: Q9BQB4-1