Osteopontin Protein (AA 17-294) (His tag)
Quick Overview for Osteopontin Protein (AA 17-294) (His tag) (ABIN7275648)
Target
See all Osteopontin (SPP1) ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 17-294
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Purification tag / Conjugate
- This Osteopontin protein is labelled with His tag.
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Purpose
- Mouse SPP1/OPN Protein
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Sequence
- Leu17-Asn294
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Characteristics
- Recombinant Mouse SPP1/OPN Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu17-Asn294.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Secreted phosphoprotein 1 (SPP1) (AA 17-300) protein (His tag)
SPP1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Secreted phosphoprotein 1 (SPP1) (AA 28-61) protein (His-IF2DI Tag)SPP1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Secreted phosphoprotein 1 (SPP1) (AA 1-300) protein (GST tag)SPP1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Secreted phosphoprotein 1 (SPP1) proteinSPP1 Origin: Human Native > 90 % (SDS-PAGE)
Secreted phosphoprotein 1 (SPP1) (AA 17-300), (partial) protein (His tag)SPP1 Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
Secreted phosphoprotein 1 (SPP1) (AA 17-287) (Active) protein (His tag)SPP1 Origin: Human Host: Escherichia coli (E. coli) > 95 % AcA, CC Active
Secreted phosphoprotein 1 (SPP1) (AA 17-317) (Active) protein (His tag)SPP1 Origin: Rat Host: HEK-293 Cells > 95 % AcA, CC Active
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
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Alternative Name
- SPP1
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Background
- Secreted phosphoprotein 1 (SPP1) expression in TAMs isolated from lung adenocarcinoma tissues and PMA-treated THP-1 cells were measured. Macrophage polarization was identified by flow cytometric analysis. Cell migration and apoptosis were assessed by Transwell migration assays and flow cytometric analysis, respectively. SPP1 is highly expressed in tumor tissues and TAMs isolated from patients with an advanced TNM stage, and also in PMA-treated THP-1 cells.
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Molecular Weight
- 31.8 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
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UniProt
- P10923
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Pathways
- Regulation of Cell Size
Target
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