SIRPG Protein (AA 29-360) (His-Avi Tag,Biotin)
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- Target See all SIRPG Proteins
- SIRPG (Signal-Regulatory Protein gamma (SIRPG))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 29-360
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SIRPG protein is labelled with His-Avi Tag,Biotin.
- Purpose
- Biotinylated Human SIRP gamma/CD172g Protein
- Sequence
- Glu29-Pro360
- Characteristics
- Recombinant Biotinylated Human SIRP gamma/CD172g Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu29-Pro360.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Anti-SIRP alpha Antibody, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human SIRP gamma, His Tag with the EC50 of 35.5ng/ml determined by ELISA. See testing image for detail.
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- Discover our top product SIRPG Protein
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Signal-Regulatory Protein gamma (SIRPG) (AA 29-360) protein (His-Avi Tag)
SIRPG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Signal-Regulatory Protein gamma (SIRPG) (AA 29-364) protein (His tag)SIRPG Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Signal-Regulatory Protein gamma (SIRPG) (AA 29-364) protein (Fc Tag)SIRPG Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
Signal-Regulatory Protein gamma (SIRPG) (AA 29-360) protein (His tag)SIRPG Origin: Human Host: Escherichia coli (E. coli) Recombinant > 80 % by SDS - PAGE SDS
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- SIRPG (Signal-Regulatory Protein gamma (SIRPG))
- Alternative Name
- SIRP gamma (SIRPG Products)
- Background
- Signal regulatory protein gamma (SIRP gamma, designated CD172g), also called SIRP beta 2, is a monomeric 45-47 kDa type I transmembrane protein belonging to the SIRP/SHPS (CD172) family of the Ig superfamily.SIRP gamma is probable immunoglobulin-like cell surface receptor. On binding with CD47, mediates cell-cell adhesion. Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation.
- Molecular Weight
- 39.7 kDa. Due to glycosylation, the protein migrates to 48-53 kDa based on Tris-Bis PAGE result.
- UniProt
- Q9P1W8
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