SIRPG Protein (His tag)

Catalog No. ABIN7317478

Product Details

Target: SIRPG (Signal-Regulatory Protein gamma (SIRPG))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This SIRPG protein is labelled with His tag.

Purpose: Recombinant Human SIRP gamma/CD172g Protein (His Tag)(Active)

Sequence: Met 1-Ser 364

Characteristics: A DNA sequence encoding the human SIRPG (NP_061026.2) extracellular domain (Met 1-Ser 364) was expressed, with a polyhistidine tag at the C-terminus.

Purity: > 96 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA.Immobilized human SIRPG-His at 10 μg/ml (100 μl/well) can bind human CD47-Fc , The EC50 of human CD47-Fc is 0.58-1.34 μg/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: SIRPG (Signal-Regulatory Protein gamma (SIRPG))

Alternative Name: SIRP gamma/CD172g (SIRPG Products)

Synonyms: CD172g Protein, SIRP-B2 Protein, SIRPB2 Protein, SIRPgamma Protein, bA77C3.1 Protein, SIRPG Protein, signal regulatory protein gamma Protein, signal-regulatory protein gamma Protein, SIRPG Protein, LOC716034 Protein, LOC102138723 Protein

Background:

Background: Signal-regulatory protein gamma (SIRPG/SIRP gamma) also known as CD172 antigen-like family member B, CD172g, and CD172g antigen, is a member of the signal-regulatory protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. SIRPG/SIRP gamma/CD172g is probable immunoglobulin-like cell surface receptor. On binding with CD47, SIRPG can mediate cell-cell adhesion. SIRPG/SIRP gamma is engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation. SIRPG/SIRP gamma/CD172g is detected in liver, and at very low levels in brain, heart, lung, pancreas, kidney, placenta and skeletal muscle. Expressed on CD4+ T-cells, CD8+ T-cells, CD56-bright natural killer (NK) cells, CD20+ cells, and all activated NK cells. This cytokine is mainly present in the paracortical T-cell area of lymph nodes, with only sparse positive cells in the mantle and in the germinal center of B-cell follicles. In the thymus, SIRPG is primarily expressed in the medulla on mature T-lymphocytes that have undergone thymic selection.

Synonym: Signal-Regulatory Protein Gamma, SIRP-Gamma, CD172 Antigen-Like Family Member B, Signal-Fegulatory Protein Beta-2, SIRP-b2, SIRP-Beta-2, CD172g, SIRPG, SIRPB2

Molecular Weight: 34.7 kDa

NCBI Accession: NP_061026