TGFB3 Protein (AA 301-412) (AVI tag,Biotin)

Catalog No. ABIN7275713

Recombinant TGFB3 protein expressed in HEK-293 Cells.

Quick Overview for TGFB3 Protein (AA 301-412) (AVI tag,Biotin) (ABIN7275713)

Target: TGFB3 (Transforming Growth Factor, beta 3 (TGFB3))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE

Product Details

Protein Characteristics: AA 301-412

Purification tag / Conjugate: This TGFB3 protein is labelled with AVI tag,Biotin.

Purpose: Biotinylated Human Mature TGF beta 3 Protein

Sequence: Ala301-Ser412

Characteristics: Recombinant Biotinylated Human Mature TGF beta 3 Protein is expressed from HEK293 with Avi tag at the C-Terminus.It contains Ala301-Ser412.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human TGF-beta RII, mFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Mature TGF beta 3, Avi Tag with the EC50 of 9.9ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 4 mM HCl.

Buffer: Lyophilized from 0.22μm filtered solution in 4 mM HCl. Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TGFB3 (Transforming Growth Factor, beta 3 (TGFB3))

Alternative Name: TGF beta 3

Background: Transforming growth factor-beta 3 is a cytokine which is involved in cell growth regulation and differentiation, stimulation of extracellular matrix and modulation of immune responses. TGF beta 3 RNA is expressed in a diverse array of tissues including perichondrium, bone, intervertebral discs, mesenteries, pleura, heart, lung, palate, and amnion, as well as in central nervous system (CNS) structures such as the meninges, choroid plexus and the olfactory bulbs. Furthermore, in several organ systems, TGF beta 3 transcripts are expressed during periods of active morphogenesis suggesting that the protein may be an important factor for the growth and differentiation of many embryonic tissues.

Molecular Weight: 14.53 kDa. Due to glycosylation, the protein migrates to 15-20 kDa based on Tris-Bis PAGE result.

UniProt: P10600-1

Pathways: Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus