TIM3 Protein (AA 22-200) (His tag)
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- Target See all TIM3 (TIM 3) Proteins
- TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 22-200
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This TIM3 protein is labelled with His tag.
- Purpose
- Human Tim-3/HAVCR2 Protein
- Sequence
- Ser22-Arg200
- Characteristics
- Recombinant Human Tim-3/HAVCR2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser22-Arg200.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human TIM-3, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-TIM-3 Antibody, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))
- Alternative Name
- Tim-3 (TIM 3 Products)
- Background
- Hepatitis A virus cellular receptor 2 (HAVCR2), also known as T-cell immunoglobulin and mucin-domain containing-3 (TIM-3), is a protein that in humans is encoded by the HAVCR2 gene.TIM3 is an immune checkpoint and together with other inhibitory receptors including programmed cell death protein 1 (PD-1) and lymphocyte activation gene 3 protein (LAG3) mediate the CD8 T-cell exhaustion. TIM3 has also been shown as a CD4 Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice.
- Molecular Weight
- 20.7 kDa, Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
- Pathways
- Regulation of Lipid Metabolism by PPARalpha, Cancer Immune Checkpoints
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