TIM3 Protein (AA 22-200) (His-Avi Tag,Biotin)

Catalog No. ABIN7275739

Product Details

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Protein Type: Recombinant

Protein Characteristics: AA 22-200

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This TIM3 protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Human Tim-3/HAVCR2 Protein

Sequence: Ser22-Arg200

Characteristics: Recombinant Biotinylated Human Tim-3/HAVCR2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser22-Arg200.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-Tim-3 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Tim-3, His Tag with the EC50 of 7.6ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Alternative Name: Tim-3 (TIM 3 Products)

Synonyms: HAVCR2 Protein, MGC140131 Protein, HAVcr-2 Protein, KIM-3 Protein, TIM3 Protein, TIMD-3 Protein, TIMD3 Protein, Tim-3 Protein, TIM-3 Protein, Tim3 Protein, Timd3 Protein, tim3 Protein, hepatitis A virus cellular receptor 2 Protein, HAVCR2 Protein, Havcr2 Protein

Background: Hepatitis A virus cellular receptor 2 (HAVCR2), also known as T-cell immunoglobulin and mucin-domain containing-3 (TIM-3), is a protein that in humans is encoded by the HAVCR2 gene.TIM3 is an immune checkpoint and together with other inhibitory receptors including programmed cell death protein 1 (PD-1) and lymphocyte activation gene 3 protein (LAG3) mediate the CD8 T-cell exhaustion. TIM3 has also been shown as a CD4 Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice.

Molecular Weight: 22.8 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result.

Pathways: Regulation of Lipid Metabolism by PPARalpha, Cancer Immune Checkpoints