TIM3 Protein (Fc Tag)

Catalog No. ABIN7320774

Product Details

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Human Cells

Purification tag / Conjugate: This TIM3 protein is labelled with Fc Tag.

Purpose: Recombinant Mouse TIM-3/HAVCR2 Protein (Fc Tag)(Active)

Sequence: Arg20-Arg191

Characteristics: Recombinant Mouse TIM-3 is produced by our Mammalian expression system and the target gene encoding Arg20-Arg191 is expressed with a Fc tag at the C-terminus.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Galectin 9-His(Cat: PKSH032470) at 2μg/ml(100 μl/well) can bind Mouse TIM-Fc. The ED50 of Mouse TIM-Fc is 4.053ug/ml .

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Alternative Name: TIM-3/HAVCR2 (TIM 3 Products)

Synonyms: HAVCR2 Protein, MGC140131 Protein, HAVcr-2 Protein, KIM-3 Protein, TIM3 Protein, TIMD-3 Protein, TIMD3 Protein, Tim-3 Protein, TIM-3 Protein, Tim3 Protein, Timd3 Protein, tim3 Protein, hepatitis A virus cellular receptor 2 Protein, HAVCR2 Protein, Havcr2 Protein

Target Type: Virus

Background:

Background: T cell immunoglobulin and mucin domain-3 (TIM3), also called hepatitis A virus cellular receptor 2 (HAVCR2), is a transmembrane glycoprotein of the TIM family of immune regulating molecules and plays an important role in the Th1-mediated immune response. TIM3 is expressed on the Th1 cells, CD8 T-cells, monocytes, and dendritic cells, but not on Th2 cells. TIM3 expressed by monocytes and dendritic cells facilitates phagocytosis of apoptotic cells and up-regulates cross-presentation of apoptotic cell-associated antigens through interaction with phosphatidylserine. Engagement of TIM3 by its ligand galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity. Stimulation of TIM3 with an agonistic antibody promotes inflammation through the activation of innate immune cells. TIM3 is also regarded as a potential target molecule for immunotherapy. TIM3 and programmed cell death 1 (PD-1) as two important coinhibitory regulators of T cell responses, have been implicated with the T-cell dysfunction or exhaustion associated with chronic HBV infection including HBV-related HCC.

Synonym: Hepatitis A virus cellular receptor 2 homolog, HAVcr-2, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, Tim3, Timd3

Molecular Weight: 46.3 kDa

UniProt: Q8VIM0

Pathways: Regulation of Lipid Metabolism by PPARalpha, Cancer Immune Checkpoints