Complement C2 Protein (His tag)

Catalog No. ABIN7447953

Recombinant Complement C2 protein expressed in HEK-293 Cells.

Quick Overview for Complement C2 Protein (His tag) (ABIN7447953)

Target: Complement C2

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: 95,00 %

Product Details

Purification tag / Conjugate: This Complement C2 protein is labelled with His tag.

Purpose: Cynomolgus Complement C2 Protein, His Tag (active enzyme)

Sequence: Ala 21 - Leu 752

Characteristics: Cynomolgus Complement C2, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Leu 752 (Accession # G8F3W0-1).

Endotoxin Level: 1.0 EU per μg

Biological Activity Comment: Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB).The specific activity is >100 pmol/min/µg (QC tested).

Application Details

Comment:

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 83.0 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris,150 mM NaCl,Ph 7.5

Storage: -20 °C

Storage Comment: -20°C

Target Details

Target: Complement C2

Background: Synonyms:C2,Complement C2,Complement Component C2,Description:C2 is a major histocompatibility complex class-III protein. Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor C4b to generate the C3 or C5 convertase. The lectin (LP) and classical (CP) pathways are two of the three main activation cascades of the complement system. These pathways start with recognition of different pathogen- or danger-associated molecular patterns and include identical steps of proteolytic activation of complement component C4, formation of the C3 proconvertase C4b2, followed by cleavage of complement component C2 within C4b2 resulting in the C3 convertase C4b2a.

Molecular Weight: 83.0 kDa

NCBI Accession: XP_005553508