Albumin Protein (ALB) (AA 25-609) (His-Avi Tag)

Catalog No. ABIN7454070

Recombinant Albumin protein expressed in HEK-293 Cells.

Quick Overview for Albumin Protein (ALB) (AA 25-609) (His-Avi Tag) (ABIN7454070)

Target: Albumin (ALB)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-609

Purification tag / Conjugate: This Albumin protein is labelled with His-Avi Tag.

Purpose: Human Serum Albumin Protein

Sequence: Asp25-Leu609

Characteristics: Recombinant Human Serum Albumin Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp25-Leu609.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: The affinity constant of 0.728 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Albumin (ALB)

Alternative Name: Serum Albumin

Background: Human serum albumin (HSA), the most prominent protein in plasma, binds different classes of ligands at multiple sites. HSA provides a depot for many compounds, affects pharmacokinetics of many drugs, holds some ligands in a strained orientation providing their metabolic modification, renders potential toxins harmless transporting them to disposal sites, accounts for most of the antioxidant capacity of human serum, and acts as a NO-carrier.

Molecular Weight: 69.4 kDa. Due to glycosylation, the protein migrates to 69-70 kDa based on Tris-Bis PAGE result.

UniProt: P02768-1

Pathways: Lipid Metabolism