IL-27 Protein (AA 21-229) (His tag)
Quick Overview for IL-27 Protein (AA 21-229) (His tag) (ABIN7454152)
Target
See all IL-27 (IL27) ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 21-229
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Purification tag / Conjugate
- This IL-27 protein is labelled with His tag.
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Purpose
- Human IL-27 Protein
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Sequence
- Arg21-Lys229(IL-27B) & Phe29-Pro243(IL-27A)
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Specificity
- Uni-Prot: Q14213 (IL-27B), Q8NEV9 (IL-27A)
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Characteristics
- Recombinant Human IL-27 Protein is expressed from CHO with His tag at the C-Terminus.It contains Arg21-Lys229(IL-27B)&Phe29-Pro243(IL-27A).
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human IL-27 RA, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 16.8ng/ml determined by ELISA (QC Test). The affinity constant of 3.58 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Interleukin 27 (IL27) (AA 1-243) protein (GST tag)
IL27 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Interleukin 27 (IL27) protein (His tag)IL27 Origin: Mouse Host: Human Cells Recombinant > 90 % as determined by SDS-PAGE
Interleukin 27 (IL27) (AA 177-243) protein (GST tag)IL27 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS, 200 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- IL-27 (IL27) (Interleukin 27 (IL27))
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Alternative Name
- IL-27
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Background
- Interleukin (IL)-27, a member of the IL-6/IL-12 family, has an important role in modulating inflammation in partnership with innate and adaptive immune cells.
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Molecular Weight
- 50.6 kDa. Due to glycosylation, the protein migrates to 54-64 kDa based on Tris-Bis PAGE result.
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UniProt
- Q14213
Target
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