IL-37 Protein (AA 27-192)

Catalog No. ABIN7454154

Recombinant IL-37 protein expressed in Escherichia coli (E. coli).

Quick Overview for IL-37 Protein (AA 27-192) (ABIN7454154)

Target: IL-37 (IL37) (Interleukin 37 (IL37))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 27-192

Purpose: Human IL-37 Protein

Sequence: Lys27-Asp192

Characteristics: Recombinant Human IL-37 Protein is expressed from E.coli without tag.It contains Lys27-Asp192.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: The affinity constant of 0.12 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in 20 mM PBS, 2 mM DTT ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL-37 (IL37) (Interleukin 37 (IL37))

Alternative Name: IL-37

Background: IL-37 is a unique member of the IL-1 family of cytokines, which functions as a natural suppressor of inflammatory and immune responses. Immune and non-immune cells produce IL-37 precursor following pro-inflammatory stimuli. Following activating cleavage by caspase-1, mature IL-37 translocates to the nucleus, where it suppresses transcription of pro-inflammatory genes.

Molecular Weight: 18.55 kDa same as Tris-Bis PAGE result.

UniProt: Q9NZH6-2