HLA-A*02:01 (AA 25-206), (Monomer) protein (His-Avi Tag,WT-1)

Catalog No. ABIN7454364

Recombinant protein expressed in HEK-293 Cells.

Quick Overview for HLA-A*02:01 (AA 25-206), (Monomer) protein (His-Avi Tag,WT-1) (ABIN7454364)

Target: HLA-A*02:01

Protein Type: Recombinant

Origin: Human, Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-206, Monomer

Purification tag / Conjugate: His-Avi Tag,WT-1

Purpose: Chimeric HLA-A*02:01 (mα3) &B2M&WT-1 (RMFPNAPYL) Monomer Protein

Sequence: Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(B2M) and RMFPNAPYL peptide

Specificity: Uni-Prot: A0A140T913 (Human HLA-A*02:01 α1, α2), P01897 (Mouse H-2Ld α3), P61769 (B2M), RMFPNAPYL

Characteristics: Recombinant Chimeric HLA-A*02:01(mα3)&B2M&WT-1 (RMFPNAPYL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr206(Human HLA-A*02:01 α1&α2)&Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119 (B2M) and RMFPNAPYL peptide.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per ug by the LAL method.

Biological Activity Comment: Immobilized Human&Mouse Chimeric WT-1 (HLA-A*02:01) , His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-WT-1 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 28.1ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: HLA-A*02:01

Background: The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A*0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A*0201 of  the MHC Class I, B2M and RMFPNAPYL peptide of  the WT-1.

Molecular Weight: 50.5 kDa. Due to glycosylation, the protein migrates to 51-60 kDa based on Tris-Bis PAGE result.

UniProt: A0A140T913