GPR77 Protein
Quick Overview for GPR77 Protein (ABIN7491551)
Target
See all GPR77 ProteinsProtein Type
Origin
Source
-
-
Purpose
- Human GPR77 full length protein-synthetic nanodisc
-
Characteristics
- Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.
-
-
Want other Options for this Protein ?
!Discover Our Predefined Custom Proteins and Custom Protein Services!ProductExpression SystemConjugateOriginPrice starts atExpression System HEK-293 CellsConjugate His tagOrigin HumanPrice starts at $16,231.82Expression System Cell-free protein synthesis (CFPS)Conjugate Strep TagOrigin HumanPrice starts at $20,480.57Your project requires further customization? Contact us and discover our custom protein solutions
-
G Protein-Coupled Receptor 77 (GPR77) (AA 1-344) protein (His tag)
custom-made GPR77 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
G Protein-Coupled Receptor 77 (GPR77) (AA 1-337) protein (His tag)custom-made GPR77 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
G Protein-Coupled Receptor 77 (GPR77) (AA 1-38) protein (Fc Tag)GPR77 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
G Protein-Coupled Receptor 77 (GPR77) (AA 1-337) protein (GST tag)GPR77 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
-
-
Comment
-
Advantages of Synthetic Nanodiscs:
- Highly purified membrane proteins
- High solubility in aqueous solutions
- High stability
- Proteins are in a native membrane environment and remain biologically active
- No detergent and can be used for cell-based assays
- No MSP backbone proteins
- Intolerant to acids and high concentrations of divalent metal ions
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Buffer
- Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
-
Storage
- -20 °C,-80 °C
-
Storage Comment
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
-
Expiry Date
- 12 months
-
-
- GPR77 (G Protein-Coupled Receptor 77 (GPR77))
-
Alternative Name
- C5AR2
-
Background
- This gene encodes a G-protein coupled receptor 1 family member involved in the complement system of the innate immune response. Unlike classical G-protein coupled receptors, the encoded protein does not associate with intracellular G-proteins. It may instead modulate signal transduction through the beta-arrestin pathway, and may alternatively act as a decoy receptor. This gene may be involved in coronary artery disease and in the pathogenesis of sepsis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]
-
Molecular Weight
- The human full length C5AR2 protein has a MW of 36.1 kDa
-
UniProt
- Q9P296
Target
-
