G Protein-Coupled Receptor 133 Protein (GPR133)

Catalog No. ABIN7491529

This Synthetic Nanodisc G Protein-Coupled Receptor 133 protein is expressed in HEK-293 Cells.

Quick Overview for G Protein-Coupled Receptor 133 Protein (GPR133) (ABIN7491529)

Target: G Protein-Coupled Receptor 133 (GPR133)

Protein Type: Synthetic Nanodisc

Origin: Human

Source: HEK-293 Cells

Product Details

Purpose: Human ADGRD1 full length protein-synthetic nanodisc

Characteristics: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Application Details

Comment:

Advantages of Synthetic Nanodiscs:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations of Synthetic Nanodiscs:

• Intolerant to acids and high concentrations of divalent metal ions

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: G Protein-Coupled Receptor 133 (GPR133)

Alternative Name: ADGRD1

Background: The adhesion G-protein-coupled receptors (GPCRs), including GPR133, are membrane-bound proteins with long N termini containing multiple domains. GPCRs, or GPRs, contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins.

Molecular Weight: The human full length ADGRD1 protein has a MW of 96.5 kDa

UniProt: Q6QNK2