GIPR Protein

Catalog No. ABIN7491651

This Synthetic Nanodisc GIPR protein is expressed in HEK-293 Cells.

Quick Overview for GIPR Protein (ABIN7491651)

Target: GIPR (Gastric Inhibitory Polypeptide Receptor (GIPR))

Protein Type: Synthetic Nanodisc

Origin: Human

Source: HEK-293 Cells

Product Details

Purpose: Human GIPR full length protein-synthetic nanodisc

Characteristics: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Application Details

Comment:

Advantages of Synthetic Nanodiscs:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations of Synthetic Nanodiscs:

• Intolerant to acids and high concentrations of divalent metal ions

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: GIPR (Gastric Inhibitory Polypeptide Receptor (GIPR))

Alternative Name: GIPR

Background: A G-protein coupled receptor for gastric inhibitory polypeptide (GIP), which was originally identified as an activity in gut extracts that inhibited gastric acid secretion and gastrin release, but subsequently was demonstrated to stimulate insulin release in the presence of elevated glucose. Mice lacking this gene exhibit higher blood glucose levels with impaired initial insulin response after oral glucose load. Defect in this gene thus may contribute to the pathogenesis of diabetes.

Molecular Weight: The human full length GIPR protein has a MW of 53.2 kDa

UniProt: P48546

Pathways: Positive Regulation of Peptide Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling