Alkaline Phosphatase Protein (ALP) (AA 20-503) (PE)
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- Target See all Alkaline Phosphatase (ALP) products
- Alkaline Phosphatase (ALP)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 20-503
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Alkaline Phosphatase protein is labelled with PE.
- Purpose
- Human Alkaline Phosphatase (Germ type) /ALPG Protein
- Sequence
- Ile20-Asp503
- Characteristics
- Recombinant Human Alkaline Phosphatase (Germ type) /ALPG Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ile20-Asp503.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Alkaline Phosphatase (ALP) (AA 23-506) protein (His tag)
ALP Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Alkaline Phosphatase (ALP) (AA 20-503) protein (His-DYKDDDDK Tag)ALP Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Alkaline Phosphatase (ALP) (AA 21-501) protein (His tag)ALP Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in 25 mM Tris, 100 mM NaCl, 1 mM MgCl2 ( pH 7.5). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Alkaline Phosphatase (ALP)
- Alternative Name
- Alkaline Phosphatase (ALP Products)
- Background
- Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
- Molecular Weight
- 79.33 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.
- UniProt
- P10696
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